GSure™ DNA Isolation Kit

GSure™ DNA isolation kit delivers high amount of DNA from minimum amount of sample source

• GSure™ DNA Extraction & Purification Kit
• GSure™ DNA Extraction & Purification Reagents
• Gel Loading Dyes
• DNA Ladder
• Gail Stain
• Running Buffer
• Agarose

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GSure™ ^Rapidplasmid DNA Mini kit

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  Features

• Fast– Isolation completes within 30 min
• Convenient- Wide range of starting culture volume (1ml-12ml)
• High yield- Recovers 3μg plasmid DNA from 1ml culture
• Reproducible- Equal amount of yield on every isolation
• Easy to use- Unique chromogenic lysis indicator sheerPink
• Quality of DNA- 260:280 absorbance ratio stays around 1.79-1.83. No contamination of protein and/or RNA and/or gDNA
• Wide Range- Can isolate plasmid sized from 2.6kb-18kb
• Downstream application- more than 75% isolated plasmid DNA comes in ccc form, best for downstream application
• Cost effective– More preps for the money

Categories

Nucleic acid Isolation and purification related products Plasmid purification related products

Applications

• Restriction digestion
• PCR amplification
• Capillary sequencing and NGS applications
• In-vitro transcription

 

Ordering Information

Cat. No.	Product Description	Pack Size	Price
G46123	GSure™  Rapidplasmid DNA Mini kit	50 Prep	$ 80.00

Product Details Specifications Protocols & Manual Product Resource

 

Description

GSure™  ^RapidPlasmid DNA Mini Kit is made for much easy, time saving and convenient way rapid for plasmid DNA isolation from 1ml culture.  Like GSure™ Splash Plasmid DNA Mini Kit, rapidPlasmid DNA mini kit is another easy to use plasmid DNA isolation kit launched globally first time by GCC Biotech India Pvt. Ltd.

^rapidPlasmid DNA mini kit is a two step method for plasmid DNA isolation. Cell harvesting step is not required; one can start from addition of first buffer directly in the culture. Mix by finger tapping followed by addition of second buffer and again mixing by tapping. That's all for the lysis. It is a silica membrane based purification strategy combined with micro spin column, making the isolation procedure much convenient and assuring the greatest level of purity of the isolated plasmid DNA molecule. This is the fastest and most convenient method of plasmid DNA isolation. From 1ml overnight grown culture, the ^rapidPlasmid DNA Mini Kit can isolate 3ug of plasmid DNA (if it is a high copy number plasmid).

Though this is a fast and most rapid method for plasmid DNA isolation, chances of getting ccc-form of plasmid is less, most of the plasmids isolate in nicked circular form. Isolation efficiency does not depend on the plasmid size (Figure 1).

The kit can isolate plasmid DNA efficiently from 0.5ml – 1.0ml overnight grown cell. Excess amount of culture inhibits the efficiency (Figure 2A and 2B). 1ml starting volume is recommended for isolation.

For elution of plasmid DNA from column, this is recommended to wait for at least 2min after adding the nuclease free water in column. Longer incubation increases the yield slightly (Figure 3).

 

GSure™  ^RapidPlasmid DNA Mini Kit

 

 

 

Materials we provide

 

Protocol & Manuals

Some product description and more information to downlode our product brochure or manual dataseet