GSure® Bacterial Genomic DNA isolation Kit
GSure® Genomic DNA Isolation Kit
GSure® DNA isolation kit delivers high amount of DNA from minimum amount of sample source
- DNA Extraction & Purification Kit
- DNA Extraction & Purification Reagents
- Gel Loading Dyes
- DNA Ladder
- Gel Stain
- Running Buffer
- Agarose
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GSure® Bacterial Genomic DNA isolation Kit
Features
- Fast – less than 45min required
- Easy – spin column format.
- Convenient- Same optimized protocol for gram positive and gram negative cells.
- High yield- Recovers ~50μg total gDNA from 3ml overnight grown E.coli cells.
- Reproducible- Delivers almost equal amount of yield on every isolation.
- Easy to use- No requirement of addition of RNase A or Proteinase K externally.
- Cost effective – More preps for the money.
- EcoFriendly- Minimum number of steps, thus minimum number of plastic ware required.
Categories
Nucleic acid Isolation and purification related products Plasmid purification related products
Advantage
- 50 μg Genomic DNA from 3ml Overnight Grown Bacterial Cells
- Extremely fast: isolation completed within 45 min.
- Total DNA isolated from the bacterial cells.
- Equally efficient on Gram positive and Gram negative cell.
- An easy to use silica membrane, spin-mini-column based DNA isolation kit for rapid, robust and reproducible isolation of genomic DNA from bacteria.
- Extremely efficient, could isolate gDNA from cells present in a colony.
- Equally efficient on stored cells.
- Maximum amount of recovery.
- Isolated DNA is compatible for all sorts of downstream application.
Ordering Information
|
Cat. No. |
Product Description |
Pack Size |
Price (₹) |
|
G46211 |
GSure® Bacterial Genomic DNA isolation Kit | 50 Prep |
Product Details Specifications Protocols & Manual Product Resource
Description
GSure® Bacterial Genomic DNA Isolation Kit can isolate total DNA from gram positive and gram negative cells. The kit isolates total DNA from the cell, genomic DNA and plasmid DNA as well. The kit is so efficient that it can isolate genomic DNA even from a single colony collected directly from the agar plate.
Genomic DNA Isolation could be done from 0.1ml to 5ml of culture. Isolated DNA is free of RNA and protein. If the cell harbors any plasmid DNA, that will also be extracted with the genomic DNA. Isolation procedure is same for gram-positive and gram-negative strains. The kit works equally efficient on bacteria having modified cell wall (slime layer/high peptidoglycan wall).
GSure® Bacterial DNA Isolation Kit contains 3 lysis buffers which assure complete enzymatic lysis of cells and digestion of contaminating proteins. Wash buffer supplied with the kit confirms complete removal of proteins and contaminants from the silica membrane. Optimized protocol guaranties extremely purified genomic DNA isolation reproducibly irrespective of the sample source.
Buffers provided with the GSure® Bacterial DNA Isolation Kit assure complete lysis of cells and digestion of cellular proteins. Buffers are already doped with RNase A and Proteinase K, thus external addition of these components are not required. All the buffers should be stored at room temperature. Prescribed volume of absolute ethanol is to be added with the wash buffer before starting the work.
GSure® Bacterial Genomic DNA Isolation Kit
Materials we provide
| Product | GSure® Bacterial Genomic DNA Isolation Kit | Competitor Q Kit |
| Number of Steps | 7 | 10 |
| Number of Buffers | 4 | 5 |
| Time Required | <45 min. | Not< 1 hr. |
| Yield | +++++ | ++ |
| Robustness | +++++ | +++ |
Protocol & Manuals
Download the product datasheet and brochure from the following links:
| Cat. No. | Product Details | Datasheet | Brochure |
| G46211 | GSure® Bacterial Genomic DNA Isolation Kit, 50 Isolation |  |
|