GSure® DNA Isolation Kit

GSure® DNA Isolation Kit

GSure®DNA isolation kit delivers high amount of DNA from minimum amount of sample source

Find support for technicalities of applications and product use
Related Products

G9 Taq DNA polymerase, 10x buffer with MgCl2
Catalogue No.: G7115
Pack Size: 1000U

Custom Oligo Synthesis

Super Stain Nucleic Acid Gel Stain,

20000X

Catalogue No.: G4708

Pack Size: 50 Preps

10x MOPS Buffer

Catalogue No.: GCR-C3

Pack Size: 100 ml

GSure® DOGMA Kit for Bacteria

Catalogue No.: GD1001

Pack Size: 50 prep

10mM dNTP Mix Ultrapure

Catalogue No.: G48

Pack Size: 1000 µl

10X TEA Buffer (Hi-Grade)

Catalogue No.: G4692

Pack Size: 1000 ml

GSure® DNA Isolation Kit

Feature

  • Convenient- Wide range of starting culture volume (1ml-12ml)
  • High yield- Recovers up to 3mg plasmid DNA from 1ml culture
  • Reproducible– Same amount of yield on every isolation
  • Easy to use- Unique chromogenic lysis indicator Sheer Pink
  • Quality of DNA- 260:280 absorbance ratio stays around 1.79-1.83. No contamination of protein and/or RNA and/or gDNA
  • Wide Range- Can isolate plasmid sized from 2.6kb-18kb
  • Downstream application- more than 75% isolated plasmid DNA comes in ccc form, best for downstream application
  • Cost effective– More preps at a low cost.

 

Categories

Nucleic acid Isolation and purification related products Plasmid purification related products

Applications

  • Restriction digestion
  • PCR amplification
  • Capillary sequencing and NGS applications
  • In-vitro transcription

 

Ordering Information

Cat.No. Product Pack Size Price in (₹)
G46121 GSure® plasmid Mini kit One Pack 50 Isolation ₹ 5,358
G4613 GSure® Plasmid Mini Kit One Pack 100 Isolation ₹ 9,125
G46131 GSure® Plasmid Mini Kit One Pack 250 Isolation ₹ 20,212
G4615 GSure® Plasmid Midi Kit One Pack 25 Isolation ₹ 17,089
G4616 GSure® plasmid Midi kit One Pack 100 Isolation ₹ 75,856
G4617 GSure® Plasmid Maxi Kit One Pack 10 Isolation ₹ 17,864
G4618 GSure® Plasmid Maxi Kit One Pack 25 Isolation ₹ 42,887
G4619 GSure® Plasmid Maxi Kit One Pack 100 Isolation ₹ 1,46,814

 

Product Details Specifications Protocols & Manual Product Resource

 

Description

GSure® Plasmid DNA Isolation Kit is an alkaline lysis based plasmid DNA isolation kit which employs silica membrane mini spin column for rapid and fast purification of the isolated DNA molecule. This kit can isolate covalently closed circular (ccc) plasmid DNA molecule from fresh overnight grown E.coli cells. Isolated plasmid is free from genomic is DNA/Protein or RNA contamination. Isolation of plasmid DNA completesd within 30 min. The kit can isolate at least 3μg plasmid DNA from 1ml of overnight grown culture of high copy number plasmid. For mid copy number plasmid, yield generally varies from 500ng-1.5μg from 1ml culture. Yield could also be enhanced by chloramphenicol. Plasmid DNA Isolation Kit can isolate GSure® plasmid DNA from 1ml to 12 ml of overnight grown cells. More than 75% of the isolated plasmid DNA is in ccc form which is suitable for any sort of downstream application. Plasmid with maximum GCC for on is isolated when 3ml culture is used beyond that the percentage of ccc from of plasmid tends to decrease.

GSure® Plasmid DNA Isolation kit can isolate different sized plasmid DNA with equal efficiency. 2.6 kb plasmid to 18kb plasmid DNA molecule has been isolated by the kit efficiently. Isolated plasmid DNA is free from genomic DNA. Spectrophotometric absorbance data reveals that the isolated plasmid DNA is free of protein and RNA contamination. A260:A280 ratio varies between 1.79-1.83. Plasmid DNA isolated by Plasmid DNA isolation kit is compatible for all sorts of GSure® downstream application like restriction digestion, PCR amplification, Sanger sequencing or in vitro transcription. GSure® Plasmid DNA Isolation kit comes with a unique chromogenic indicator, sheerPink. SheerPink helps to ensure completion of lysis reaction. A complete lysis and proper neutralization assures ccc form of isolated plasmid with no genomic DNA contamination. Plasmid DNA Isolation Kit GSure® contains 3 buffers which assure complete lysis of cells and digestion of contaminating proteins. Wash buffer supplied with the kit ensures complete removal of residual salts to from the silica membrane. Optimized protocol guaranties extremely purified plasmid DNA isolation reproducibly. Prescribed volume of absolute ethanol is to be added to the wash buffer before starting the workFast– Isolation completes within 30 min

 

GSure® DNA Isolation Kit

 



Specifications

Materials we provide
Product Size 500U & 2500U
Shipping Condition: Dry Ice

 

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Cat.No. Product Datasheet Brochure
G46121 GSure® plasmid Mini kit One Pack
G4613 GSure® Plasmid Mini Kit One Pack
G46131 GSure® Plasmid Mini Kit One Pack
G4615 GSure® Plasmid Midi Kit One Pack
G4616 GSure® plasmid Midi kit One Pack
G4617 GSure® Plasmid Maxi Kit One Pack
G4618 GSure® Plasmid Maxi Kit One Pack
G4619 GSure® Plasmid Maxi Kit One Pack