Fastract® Direct PCR Kit for Cultured Cell
Fastract® Direct PCR Kit
The kit employs a specially engineered high fidelity DNA
Polymerase enzyme that exhibits high resistance to many PCR inhibitors
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Fastract® Direct PCR Kit for Cultured Cell
Guidelines of Sample Preparation
- When working with cultured cell, use maximum 1 million or as least as 10000 cells.
- The cells must be pellet down and must be washed with 1X PBS solutions 2-3 times.
- The cells must be suspended in 1X PBS solution
- Collect 25µl of this cell suspension in a PCR tube, add 100µl Fastract® Cultured cell Lysis Buffer into each tubes, vortex well and incubate them at 95°C for 15 mins.
- After incubation centrifuge the PCR tubes at 15000 rpm for 2 mins. Collect the clear supernatant in a separate microfuge tube.
- Use 1.5µl of this clear lysate as template for the PCR reaction.
Troubleshooting
- In case of no PCR reaction dilute the cell lysate 1:10 or 1:50 with Fastract® cultured cell lysis buffer and use 1.5µl of this dilution as template.
- In case of non-specific amplification, increase the annealing temperature, reduce the total number of cycles or design a new set of primers.
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G45313A | FasTract Direct PCR Kit® for Cultured Cell | 100 rxn | POR |
| G45313 | FasTract Direct PCR Kit® for Cultured Cell | 500 rxn | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
FasTract Cultured cell Direct PCR Kit is designed to perform PCR directly from cultured cell without prior DNA purification. Fresh or frozen cells all are suitable templates for this kit. The kit employs a specially engineered high fidelity DNA Polymerase enzyme that exhibits high resistance to many PCR inhibitors found in cell or tissue culture media. The kit ensures high yield and is time saving as DNA isolation from samples can be avoided prior to PCR. The kit is recommended for end-point PCR.
FasTract® Direct PCR Kit for Cultured Cell
Specifications
Kit Component
- FasTract® Cell Lysis Buffer
- FasTract® Tissue 1X PCR Mix (FasTract® DNA Polymerase, Reaction buffer, dNTP (10mM))
- Control Primer Mix (10mM each)
- Carefully mix and spin down all tubes before opening to ensure homogeneity and improve recovery. The PCR setup can be performed at room temperature.
- Always add the Blood lysate last to the reaction.
- Always ensure that the volume of lysate that is being added to the PCR reaction is 3% of the total reaction volume. Do not add more lysate to the reaction mix.
Shipping and Storage
FasTract® Direct PCR Kit is shipped on gel ice. Upon arrival, store the components at 4°C. Do not freeze.
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G45313A | FasTract Direct PCR Kit® for Cultured Cell, 100 rxn |  |
|
| G45313 | F FasTract Direct PCR Kit® for Cultured Cell, 500 rxn | |
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