FasTract® Direct PCR Kit Forensic Samples

Fastract® Direct PCR Kit

The kit employs a specially engineered high fidelity DNA
Polymerase enzyme that exhibits high resistance to many PCR inhibitors

Find support for technicalities of applications and product use

Related Products

100bp DNA Step Ladder

Catalogue No. : G4667

Pack Size: 50 Preps

Custom Oligo Synthesis

Fastract® Direct PCR Kit for Forensic Samples

Guidelines of Sample Preparation

Blood stored with anticoagulants, clotted blood and even blood spots on paper discs, all are suitable samples for this FasTract® Forensic Direct PCR kit.
When working with clotted blood or blood, 10-20 µl blood is recommended.
In case of spotted blood on paper discs use a single hole paper puncher to punch out a spotted blood disc. This will be the sample.
Saliva or any other swab samples collected in swab sticks all are suitable samples for this Fastract Forensic Direct PCR kit.
Scalp and body hair all are suitable samples for this Fastract Forensic Direct PCR kit.
Collect the samples in a PCR tube ,add 100 µl Fastract Forensic Lysis Buffer into each tubes, vortex well and incubate them at 95°C for 15 mins.
After incubation centrifuge the PCR tubes at 15000 rpm for 2 mins. Collect the clear supernatant in a separate microfuge tube
Use 1. 5µl of this clear lysate as template for the PCR reaction.

Guidelines for PCR Setup

Add 47.5µl 1X Fastract Tissueu cell Direct PCR mix in a Flat Capped PCR tube
Add 0.5 µl of each Primers (from 10mM stock) to the master mix
Use1. 5 µl of Fastract cultured cell lysate as template for the PCR reaction
While setting up the PCR cycle keep the initial denaturation time to be 5 mins at 95°C followed by denaturation at 95°C for 10 -15 sec and annealing at the desired Tm. For extension, use 1min for 1Kb amplicon size.

Troubleshooting

In case of no PCR reaction dilute the tissue lysate 1:10 or 1:50 with Fastract Tissue lysis buffer and use 1.5 μl of this dilution as template
In case of non-specific amplification, increase the annealing temperature, reduce the total number of cycles or design a new set of primers.

Ordering Information

Cat. No.	Product	Pack Size	Price
G45316A	Fastract Direct PCR Kit® for Forensic Samples	100 rxn	POR
G45316	Fastract Direct PCR Kit® for Forensic Samples	500 rxn	POR

Product Details Specifications Protocols & Manual Product Resource

Description

Fastract® Direct PCR Kit is designed to perform PCR directly from hair blood, and saliva without prior DNA purification. Freshly collected blood, blood stored with anticoagulants, clotted blood and even blood spots on paper discs, all are suitable samples for this Fastract® Direct PCR kit. The kit employs a specially engineered high fidelity DNA Polymerase enzyme that exhibits high resistance to many PCR inhibitors found in blood and other biofluids. The kit ensures high yield and is time saving as DNA isolation from samples can be avoided prior to PCR. The kit is recommended for end-point PCR.

Fastract® Direct PCR Kit for Forensic Samples

Specifications

Kit Component

Fastract® Lysis Buffer
Fastract® 1X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP (10mM))
Control Primer Mix (10mM each)

Important Notes

Carefully mix and spin down all tubes before opening to ensure homogeneity and improve recovery. The PCR setup can be performed at room temperature.
Always add the Tissue sample lysate last to the reaction.
Always ensure that the volume of lysate that is being added to the PCR reaction is 3% of the total reaction volume. Do not add more lysate to the reaction mix

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Catalogue No.	Product Details	Datasheet	Brochure
G45316A	FasTract® Direct PCR Kit for Forensic Samples, 100 rxn
G45316	FasTract® Direct PCR Kit for Forensic Samples, 500 rxn