Fastract® Direct PCR Kit for Rice
Fastract® Direct PCR Kit
The kit employs Rice Direct PCR Kit for direct PCR from
rice leaves without isolation of total genomic DNA. & validated over different varieties of rice and
analyzed using 50 SSR genetic markers
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Find support for technicalities of applications and product use
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Fastract® Direct PCR Kit for Rice
Highlights
- Direct PCR- sample is added directly to PCR reactions therefore there is no need for time consuming and expensive DNA purification steps.
- Storage protocol available - allows multiple PCR reactions from one tiny sample and allows re-testing.
- Specially engineered Hi-Fidelity Fastract® DNA polymerase—extremely short PCR protocol times.
- Master Mix format with premixed gel loading dye- minimizes possibility of cross contamination, reduces sample handling and allows direct loading on gel.
Applications
- Genotyping
- Transgene detection
- Sequencing
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G45322 | Fastract Direct PCR Kit® for Rice (50 RM markers) | 1000 rxn | POR |
| G45322A | Fastract Direct PCR Kit® for Rice (50 RM markers) | 10000 rxn | POR |
| G45322CK | Fastract® Direct Rice Screening Core Kit (50 RM markers)(100samples) | 1 Kit | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
Molecular markers are now widely used to track loci and genome regions in several crop-breeding programmes. PCR-based markers analysis and gene expression studies are increasing exponentially. In these studies high-quality and intact DNA is the keystone for performing PCR based research. However, individual species of plants can behave differently during extraction due to difference in metabolic activities.
Rice (Oryza sativa L.) synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA. These polysaccharides co-precipitate during DNA extraction and also known to inhibit polymerase activity.
Fastract® Rice Direct PCR Kit provides ease for direct PCR from Rice leaves without isolation of total genomic DNA. It is an improved version of GCC Biotech Fastract® Plant Direct PCR Kit. This newly improved and optimized version of FasTract® Direct PCR Kit has been validated over different varieties of rice and analyzed using 50 SSR genetic markers, distributed across 12 rice chromosomes reported in GRAMENE website.
Simple sequence repeat (SSR) is one of the most robust marker for identifying rice varieties for assessment of genetic diversity and population structure.
Specifications
|
Marker Name |
Chromosome No. | Volume of Lysis Buffer (ml) | Incubation Time
|
PCR Cycles | Optimized Tm (0C) | Amplicon size (base pairs) |
Type of Amplification |
|
Rm 495 Rm 1 Rm 283 Rm 259 Rm 312 Rm 5 Rm 237 Rm 431 Rm 154 Rm 452 Rm 489 OSR 13 Rm 338 Rm 55 Rm 514 Rm 307 Rm 124 Rm 507 Rm 413 Rm 161 Rm 178 Rm 334 Rm 133 Rm 510 Rm 454 Rm 162 Rm 125 Rm 11 Rm 455 Rm 118 Rm 408 Rm 152 Rm 25 Rm 44 Rm 284 Rm 433 Rm 447 Rm 316 m 105 Rm 215 Rm 474 Rm 271 Rm 171 Rm 484 Rm 552 Rm 536 Rm 287 Rm 144 Rm 19 Rm 277 |
1
1 1 1 1 1 1 1 2 2 3 3 3 3 3 4 4 5 5 5 5 5 6 6 6 6 7 7 7 7 8 8 8 8 8 8 8 9 9 9 10 10 10 10 11 11 11 11 12 12 |
30
30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 |
15 Mins
15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins 15 Mins |
40
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 |
54
52 58 54 50 54 58 58 66 52 52 58 60 54 56 54 64 56 54 62 62 58 64 58 58 65 65 56 52 62 58 52 56 48 60 58 60 56 65 58 56 50 58 58 56 56 50 64 52 54 |
159
78 156 172 >500 113 133 251 183 209 271 99 184 >500 245 129 271 258 79 >500 117 >500 230 122 >500 229 >500 126 131 159 129 152 141 >500 148 312 111 197 134 150 252 >500 329 299 195 243 107 225 >500 117 |
Intense Moderate Intense Intense Moderate Moderate Intense Intense Intense Intense Intense Moderate Moderate Intense Intense Faint Intense Intense Moderate Faint Intense Faint Intense Intense Moderate Intense Moderate Intense Intense Intense Intense Intense Faint Faint Intense Intense Intense Intense Moderate Intense Faint Moderate Intense Moderate Faint Intense Faint Intense Moderate Intense |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G45322 | Fastract® Direct PCR Kit for Rice (50 RM markers), 1000 rxn |  |
|
| G45322A | Fastract® Direct PCR Kit for Rice (50 RM markers), 10000 rxn | |
|
| G45322CK | Fastract® Direct Rice Screening Core Kit (50 RM markers)(100samples), 1 Kit | |
|