Dual Step RT-PCR Kit

 Dual Step RT-PCR Kit


Features

  • High cDNA yields even from low-abundance transcripts
  • Multiple transcripts can be detected from asingle first strand cDNA synthesis
  • Semi quantitative analysis of the mRNA level can be achieved by agarose gel electrophoresis

 

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Repeatative freeze-thaw cycles should be minimized.

 

Ordering Information

Cat. No.

Product Pack Size Price

G7114

Dual Step RT-PCR KitM-Mulv RT(lOOOOu), 10X RT Buffer (IX 125 pi), Oligo (dT) primer (1X50 pi), oligo hexamer (50 pi), dNTP mix, 10 mM mix (IX 50 pi), Ribonuclease Inhibitor (50pl), Sterile, DEPC water) 1.5 ML) 50 rxn

POR

G7114A Dual Step RT-PCR KitM-Mulv RT(10000u), 10X RT Buffer (IX 125 pi), Oligo (dT) primer (1 X 50 pi), oligo hexamer (50 pi), dNTP mix, 10 mM mix (IX 50 pi), Ribonuclease 100 rxn

POR

 


Product Details Specifications Protocols & Manual Product Resource

 

Description

GCC Biotech offers Dual Step RT-PCR Kit for fast cDNA synthesis enabling two-step RTPCR for gene expression analysis. In the first step, M-MulV Reverse Transcriptase (RT) is used with random primer, oligodT primer or gene specific primer annealed to an RNA sample. In the second step, PCR amplification is performed in a separate tube using gene specific primers. A ready to useTaq 2X PCR Master Mix is provided for its convenient and consistent amplification performance.

 

Dual Step RT-PCR Kit

 

 

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Catalogue No. Product Details Datasheet Brochure
G7114 Dual Step RT-PCR KitM-Mulv RT(lOOOOu), 10X RT Buffer (IX 125 pi), Oligo (dT) primer (1X50 pi), oligo hexamer (50 pi), dNTP mix, 10 mM mix (IX 50 pi), Ribonuclease Inhibitor (50pl), Sterile, DEPC water) 1.5 ML)
G7114A Dual Step RT-PCR KitM-Mulv RT(10000u), 10X RT Buffer (IX 125 pi), Oligo (dT) primer (1 X 50 pi), oligo hexamer (50 pi), dNTP mix, 10 mM mix (IX 50 pi), Ribonuclease