GPfu PCR Master Mix
Thermostable DNA polymerases
Thermostable DNA polymerases
- DNA Extraction & Purification Kit
- DNA Extraction & Purification Reagents
- Gel Loading Dyes
- DNA Ladder
- Gel Stain
- Running Buffer
- Agarose
Find support for technicalities of applications and product use
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GPfu PCR Master Mix
Features
- Easy to use for long range PCR
- For sensitive high throughput PCR
- Tolerates a wide range of genomic DNA templates
- Generates PCR products with 3'-dA overhangs
Applications
- High Fidelity PCR can amplify up to 6 kb
- Blunt-end PCR Cloning or mutagenesis requested high fidelity
- Site directed mutagenesis
Unit definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmoles of dNTP into acid insoluble material in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze / thawing of reagents.
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G7122 | GPfu PCR Master Mix | 100 rxn | POR |
| G7122A | GPfu PCR Master Mix | 200 rxn | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
Gpfu PCR Master Mix is a thermostable DNA polymerase isolated from hyperthermophilic Pyrococcus furiosus. The protein is isolated from a recombinant E.coli strain containing the gene encoding Pfu DNA polymerase. It catalyzes the DNAdependent polymerization of nucleotides into duplex DNA in the 5' → 3' direction and exhibits 3' → 5' exonuclease (proof reading) activity. Pfu DNA polymerase is the ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by PCR reaction. It can apply to cloning, gene expression, site-directed mutagenesis and etc.
GPfu PCR Master Mix
|
Component |
G7122 | G7122A |
|
GPfu PCR Master Mix (2.5 unit/λ)10X 10X GPfu Buffer 25 mM MgSO4 Control DNA template Control Primer mix |
100 Unit (2 X 0.5 ML) (2 X 0.5 ML) 10 µl 10 µl |
2X100 Unit (4 X 0.5 ML) (4 X 0.5 ML) 10 µl 10 µl |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G7122 | GPfu PCR Master Mix, |  |
|
| G7122A | GPfu PCR Master Mix, | |
|