G9 Taq DNA Polymerase
Thermostable DNA polymerases
Thermostable DNA polymerases
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Find support for technicalities of applications and product use
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G9 Taq DNA Polymerase
Features
- Designed to be compatible with existing assay systems
- Unique buffer formulation facilitates specific primer annealing, leading to higher yield of specific product
- Half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
Application
- High throughput PCR from complex genomic, viral, and plasmid templates
- TA cloning
- RT-PCR
Unit definition
One unit of G9 Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C material in 30 min at 74°C
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze/thawing of reagents.
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G7115B | G9 Taq DNA polymerase, 10x buffer with MgCl2 | 500 U | POR |
| G7115 | G9 Taq DNA polymerase, 10x buffer with MgCl2 | 1000 U | POR |
| G7115A | G9 Taq DNA polymerase, 10x buffer with MgCl2 | 5000 U | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
G9 Taq DNA Polymerase is a thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. This recombinant protein was expressed and purified from E.coli. This Polymerase catalyzes the polymerization of nucleotides into duplex DNA in 5'- 3' direction in the presence of magnesium ions , has no detectable 3'— > 5' exonuclease (proofreading) activity and possesses low 5'— >3' exonuclease activity. In addition, G9 Taq DNA polymerase exhibits template independent single molecule deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Thus, the PCR amplified product using G9 Taq DNA Polymerase is ready for TA cloning. G9 Taq DNA Polymerase is the ideal tool for standard PCR of templates 6 kb or shorter.
G9 Taq DNA Polymerase
|
Component |
G7115B | G7115 |
G7115A |
|
Taq (2.5 unit//λ) 10XTaq Buffer 25 mM MgCl2 Control DNA template Control Primer |
500Unit
(2 X 1.25 ml) (2 X 1.25 ml) 10 μl 10 μl |
2X500Unit
(2 X 1.25 ml) (2 X 1.25 ml) 10 μl 10 μl |
10X500Unit
(20 X 1.25 ml) (20 X 1.25 ml) 10 μl 10 μl |
| Price | 2,109 | 3,403 |
12,717 |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G7115 | G9 Taq DNA polymerase, 10x buffer with MgCl2, 1000 U |  |
|
| G7115A | G9 Taq DNA polymerase, 10x buffer with MgCl2, 5000 U | |
|
| G7115B | G9 Taq DNA polymerase, 10x buffer with MgCl2, 500 U | |
|