Hi-G9Taq DNA Polymerase
Thermostable DNA polymerases
Thermostable DNA polymerases
- GSure™ Ultra Nucleic Acid Kit for DNA & RNA Isolation
- GSure™ Dogma kit for DNA, RNA & Protein Isolation
- Reverse Transcription & cDNA Synthesis
- Thermostable DNA Polymerases
- Fastract™Direct PCR Kit
- SYBR green & Taqman based qRT-PCR Kits
- TA-Cloning kit and Related Enzymes
- Competent Cells
- Site Directed Mutagenesis Based Kits
- Sequencing Grade Reagents
Find support for technicalities of applications and product use
Related Products
Catalogue No. :G4629A
Pack Size: 50 Preps
Catalogue No. : G4667
Pack Size: 50 Preps
Hi-G9 Taq DNA Polymerase
Features
- Improved sensitivity, specificity
- High yields
- Unique buffer formulation facilitates high throughput PCR
- PCR for higher fragment upto 8 kb
- Generates PCR products with 3'-dA overhangs
Application
- High throughput PCR from complex genomic, viral, and plasmid templates
- TA cloning
- PCR for high GC content amplicons
- RT-PCR
Unit definition
One unit of Hi-G9 Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze/thawing of reagents
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| GG03 | Hi- G9 Taq DNA polymerase | 500 U | POR |
| GG01 | Hi- G9 Taq DNA polymerase | 1000 U | POR |
| GG02 | Hi- G9 Taq DNA polymerase | 5000 U | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
Hi-G9 Taq DNA Polymerase is a thermostable recombinant DNA polymerase, expressed and purified from E.coli. Like G9 polymerase this polymerase catalyzes the polymerization of nucleotides into duplex DNA in 5'- 3' direction in the presence of magnesium ions , has no detectable 3'→ 5' exonuclease (proofreading) activity and possesses low 5'→ 3' exonuclease activity. Hi-G9 Taq DNA polymerase is G9 Taq DNA polymerase supplied with a special and improved buffer system that increases thermostability (enzyme half-life) and processivity of Taq Polymerase by stabilizing the enzyme during PCR. Hi-G9 Taq DNA Polymerase buffer is a combination of different additives, which protects enzymes under stress conditions such as desiccation, heat, and changes in pH and salt concentration. Hi-G9 Taq polymerase shows a robust amplification of templates even with higher complexity.
Hi-G9 Taq DNA Polymerase
|
Component |
GG03 | GG01 |
GG02 |
|
Taq (2.5 unit/λ) 10 X Hi-G9 Taq Buffer 25 mM MgCl2 10 X GCfix Buffer Control DNA template Control Primer mix |
500Unit
(2 X 1.25 ML) (2 X 1.25 ML) (2 X 1.25 ML) 10 μl 10 μl |
2X500Unit
(4 X 1.25 ML) (4 X 1.25 ML) (4 X 1.25 ML) 10 μl 10 μl |
10X500Unit
(20 X 1.25 ML) (20 X 1.25 ML) (20 X 1.25 ML) 10 μl 10 μl |
| Price | 32.19 | 57.00 |
247.00 |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| GG03 | Hi- G9 Taq DNA polymerase, 500 U |  |
|
| GG01 | Hi- G9 Taq DNA polymerase, 1000 U | |
|
| GG02 | Hi- G9 Taq DNA polymerase, 5000 U | |
|