Hi-Prime Taq DNA Polymerase
Thermostable DNA polymerases
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Hi-Prime Taq DNA Polymerase
Features
- A perfect blend of two different DNA polymerases for the robust yield in PCR reaction of higher sized fragments
- Unique buffer formulation to facilitate improved sensitivity, specificity, and yields for long range PCR ( ≥ 12 Kb)
Application
- PCR from natural isolates.
- PCR for diagnostic samples.
- PCR for forensic samples.
- PCR for high GC content amplicons
- PCR for soil DNA
Unit definition
One unit of Prime Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze/thawing of reagents.
Heat Inactivation- 3’ to 5’ Exonuclease activity- Yes, 5’ to 3’ Exonuclease activity- Yes
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G7116 | Hi-Prime Taq DNA Polymerase | 500 U | POR |
| G7116A | Hi-Prime Taq DNA Polymerase | 1000 U | POR |
| G7116B | Hi-Prime Taq DNA Polymerase | 5000 U | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
Hi-Prime Taq DNA Polymerase is an optimized blend of G9 Taq DNA polymerase and high fidelity DNA polymerases from Pyrococcus species in presence of specially formulated buffer which supports PCR amplification from critical amplicons. Presence of high fidelity DNA polymerases from Pyrococcus species in optimized ratio and the enhancer buffer system make Hi-Prime Taq DNA polymerase an excellent choice for PCR amplification from natural isolates. The 3´→ 5´ exonuclease activity of the high fidelity DNA Polymerase increases the fidelity and robustness in amplification by Taq DNA Polymerase, even from very low copy number of template.
Hi-Prime Taq DNA polymerase comes with a specially formulated buffer, which allows amplification up to 12kb or more.
|
Component |
G7116 | G7116A |
G7116B |
|
Taq (2.5 unit/λ) 10X Prime Taq Reaction Buffer 25 mM MgCl2 Control DNA template Control Primer mix |
500Unit
(2 X 1.25 ML) (2 X 1.25 ML) 10 μl 10 μl |
2X500Unit
(4 X 1.25 ML) (4 X 1.25 ML) 10 μl 10 μl |
10X500Unit
(20 X 1.25 ML) (20 X 1.25 ML) 10 μl 10 μl |
| Price in ₹ | POR | POR |
POR |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Produst Details | Datasheet | Brochure |
| G7116 | Hi-Prime Taq DNA Polymerase, 500 U |  |
|
| G7116A | Hi-Prime Taq DNA Polymerase, 1000 U | |
|
| G7116B | Hi-Prime Taq DNA Polymerase, 5000 U | |
|