HotStart Taq DNA Polymerase
Thermostable DNA polymerases
Thermostable DNA polymerases
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HotStart Taq DNA Polymerase
Features
- Inactive at room temperature and activated at elevated temperature (95⁰C)
- Only five minutes activation time
- High PCR specificity and sensitivity with minimal optimization
- Room temperature PCR set up
- Reversible aptamer based inactivation
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months. when stored properly. Avoid repeated freeze/thawing of reagents.
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G7120 | HotStart Taq DNA Polymerase | 100 u | POR |
| G7120A | HotStart Taq DNA Polymerase | 500 u | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
HotStart Taq DNA polymerase from GCC Biotech India Pvt. Ltd. is an aptamer based hotstart Taq DNA Polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Suboptimal activity of Taq DNA polymerase at room temperature usually leads to nonspecific amplification in PCR reaction. In GCC Biotech HotStart Taq DNA polymerase, the aptamer-inhibitor reversibly binds to the active site of the enzyme Oand inhibits the activity of the polymerase at room temperature (up to 45 C), thus avoiding any extension due to non-specific annealing of the primers at room temperature, thereby generating more cleaner and specific PCR product . The Hotstart Taq DNA polymerase is improved to have a faster activation time and higher half-life. This aptamer-based hot start does not need any separate high temperature step to Oactivate the enzyme and under standard hot-start conditions (i.e., 95 C in pH 7.8), HotStart Taq recovers its 5'-3' polymerizing activity within 5 minutes, thus allowing the reactions to be set up at room temperature. HotStart Taq is also superior to antibodybased hot-start DNA polymerases due to complete supression of enzyme activity at room temperature and attainment of full enzyme activity following the activation step. Codon optimized Taq DNA Polymerase gene of Thermus aquaticus was cloned and purified from E. coli host. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of primer. The formulation of this enzyme is changed in such a way so as to improve its activity for longer amplification. It can be used for PCR of up to 6 kb product. Thus generating a cleaner and specific amplification.
HotStart Taq DNA Polymerase is provided with 10x HS Taq reaction buffer and 25mM MgCl2 The buffer does not contain any MgCl2
|
Initial Denaturation |
Denaturation | Annealing | Extention |
Final Extension |
Store |
| 90°C
5 mins 1 Cycles |
90°C
15 mins |
X0C
15-30 sec |
68-720C
1 min/ 1 kb |
68-720C
7-30 mins 1 cycle |
40C a 1 cycle |
|
25-35 cycle |
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Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G7120 | HotStart Taq DNA Polymerase, 100 u |  |
|
| G7120A | HotStart Taq DNA Polymerase, 500 u | |
|