Prime Taq DNA Polymerase
Thermostable DNA polymerases
Thermostable DNA polymerases
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Find support for technicalities of applications and product use
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Prime Taq DNA Polymerase
Features
- A perfect blend of two different DNA polymerases for the robust yield in PCR reaction of higher sized fragments.
- Unique buffer formulation to facilitate improved sensitivity, specificity, and yields for long range
- Robust yield of long amplicons (up to 10 kb) as compared to any Taq DNA polymerase.
Application
- High throughput Long range PCR from complex genomic, viral, and plasmid templates (up to 10 kb)
- Colony PCR
- GC-rich PCR
- AT-rich PCR
- Routine use PCR
- RT-PCR
Unit definition
One unit of Prime Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze/thawing of reagents.
Heat Inactivation- 3’ to 5’ Exonuclease activity- Yes, 5’ to 3’ Exonuclease activity- Yes
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| G4798 | Prime Taq DNA Polymerase | 500 U | POR |
| G4798A | Prime Taq DNA Polymerase | 1000 U | POR |
| G4799 | Prime Taq DNA Polymerase | 5000 U | POR |
Product Details Specifications Protocols & Manual Product Resource
Description
Prime Taq DNA Polymerase is an optimized combination of G9 Taq DNA polymerase and high fidelity DNA polymerases from Pyrococcus species for use in routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of the high fidelity DNA Polymerase increases the fidelity and robustness in amplification by Taq DNA Polymerase, even from very low copy number of template. The 10 X Reaction Buffer has also been formulated for robust yield of desirable PCR products with requirement of minimal optimization. Prime Taq DNA polymerase is capable of amplifying amplicons up to 10kb. Prime Taq DNA polymerase has improved efficiency of polymerization irrespective of templates having high GC content and contamination of exogenous PCR inhibitor. Prime Taq DNA Polymerase is supplied with 10X Prime Taq Standard Reaction Buffer to provide robust amplification.
Prime Taq DNA Polymerase
|
Component |
G4798 | G4798A |
G4799 |
|
Taq (1 unit/µl) 10X Prime Taq Reaction Buffer 25 mM MgCl2 Control DNA template Control Primer mix |
500Unit
(2 X 1.25 ML) (2 X 1.25 ML) 10 μl 10 μl |
2X500Unit
(2 X 1.25 ML) (2 X 1.25 ML) 10 μl 10 μl |
10X500Unit
(20 X 1.25 ML) (20 X 1.25 ML) 10 μl 10 μl |
| Price in ₹ | 6,031 | 21,850 |
40,288 |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| G4798 | Prime Taq DNA Polymerase, 500 U |  |
|
| G4798A | Prime Taq DNA Polymerase, 1000 U | |
|
| G4799 | Prime Taq DNA Polymerase, 5000 U | |
|