Thermostable DNA polymerases

Thermostable DNA polymerases

 

• GSure™  Ultra Nucleic Acid Kit for DNA & RNA Isolation
• GSure™ Dogma kit for DNA, RNA & Protein Isolation
• Reverse Transcription & cDNA Synthesis
• Thermostable DNA Polymerases
• Fastract™Direct PCR Kit
• SYBR green & Taqman based qRT-PCR Kits
• TA-Cloning kit and Related Enzymes
• Competent Cells
• Site Directed Mutagenesis Based Kits
• Sequencing Grade Reagents

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Find support for technicalities of applications and product use

 

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  Features

• Designed to be compatible with existing assay systems
• Amplify from very low amount of template
• Resistant to PCR Inhibitors, can amplify in presence of blood, serum and urine
• Generates PCR products with 3′-dA overhangs

 

Applications

• PCR for diagonistic samples
• PCR for forensic sample
• PCR for GC rich amplicon
• RT-PCR
• qPCR

 

Unit definition

One unit of DeltaQ Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

 

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. Avoid repeated freeze/thawing of reagents.

 

 

Ordering Information

Cat. No.	Product	Pack Size	Price
G7126	DaltaQ Polymerase	250 rxn	₹ 9,900
G7126A	DaltaQ Polymerase	(4X250) rxn	₹ 31,276

 

Product Details Specifications Protocols & Manual Product Resource

 

Description

DeltaQ polymerase is a truncated version of Taq DNA Polymerase, lacking the first 278 amino acids. To enhance its DNA binding affinity a dsDNA binding domain protein has been fused with this truncated polymerase. Normally DeltaQ polymerase is able to amplify upto 4 kb from lambda DNA. Its enhanced binding property endow It to amplify from very low amount template (~ fmole level). DeltaQ polymerase also contains mutations in its polymerase domain that make it resistant to inhibitors present in body fluid. This resistant nature to pcr inhibitors, makes DeltaQ polymerase a suitable choice for PCR from whole blood , serum, urine. DeltaQ polymerase tolerates up to 15% whole blood and 50-60% blood serum in a 50 μl reaction which make it unique in its character from rest of the available polymerases. Apart from this special characteristics DeltaQ polymerase can amplify from high GC rich template.

 

 

DaltaQ Polymerase

 

 

 

 

Component	G7126	G7126A
DeltaQ Polymerase (2.5 unit/λ)  10X DeltaQ Buffer  30 mM MgCl2  Gcfix cocktail  Control DNA template  Control Primer mix	100 Unit (2 X 0.5 ML) (2 X 0.5 ML) 10 µl 10 µl	2X100 Unit (4 X 0.5 ML) (4 X 0.5 ML) 10 µl 10 µl

 

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Catalogue No.	Product Details	Datasheet	Brochure
G7126	DaltaQ Polymerase, 250 u
G7126A	DaltaQ Polymerase, (4X250) 1000 u