Max MMLV Reverse Transcriptase

Max MMLV Reverse Transcriptase

Covid-19 Detection Product


Find support for technicalities of applications and product use

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Max MMLV Reverse Transcriptase


Features

  • First-strand synthesis of cDNA from RNA molecules
  • cDNA synthesis with long messenger RNA templates (>5kb)
  • RNaseH minus recombinant MMLV RT is the preferred reverse transcriptase for long mRNA templates.
  • A truly thermostable reverse transcriptase for fast synthesis of short cDNA (< 1 Kb)
  • Extremely active at high temperatures (55 to 80°C): Improves specificity of cDNA Synthesis from diverse RNA templates
  • Sensitive: Detects ≤100 copies of RNA in two-step RT-qPCR assays
  • Short reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results
  • Batch to batch reproducibility: Manufactured in an ISO 13485-certified facility
  • onsistent, reliable amplification helps provide results you can trust

 

Specifications

  • Storage: Store at -20°C.
  • Storage Buffer: The enzyme is supplied in: 50mM Tris-HCl (pH 7.6), 150mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% (v/v) Nonidet P40, 50% glycerol (v/v).
  • Unit Definition: One unit incorporates 1 nmole of dTTP into acid-precipitable material in 10 min at 37°C using poly(A)•oligo(dT) as template primer.
  • Endonuclease: Free of contaminating endonucleases at the 10 Unit level.
  • Exonuclease: Free of contaminating exonucleases at the 10 Unit level.
  • RNase Activity: No RNase activity detected using a fluorescence-based RNase activity assay.

 

 Ordering Information

Cat. No. Product Description Size Price
G4645 Max M-MLV Reverse Transcriptase RNaseH minus (Concentration: 200U/ul) 10000 U POR
G4646 Max M-MLV Reverse Transcriptase RNaseH minus (Concentration: 200U/ul) 50000 U POR

 


Product Details Specifications Protocols & Manual Product Resource

 

Description

One-Step A reverse transcriptase (RT) is a DNA polymerase enzyme that synthesises cDNA from an RNA template. RTs are widely used to study gene expression in cells or tissues, in next-generation sequencing (NGS) applications, and in conjunction with quantitative PCR (qPCR) or isothermal amplification methods to detect and identify RNAs that are of clinical or functional significance.
Known limitations in reverse transcription enzymes (RTs) compromise current RT PCR and RNA sequence analyses. Most common RT protocols use retroviral RTs that impair analysis due to low accuracy, strand switching and bias. Since none of these RT enzymes are truly thermostable, depending upon this need we GCC Biotech’s research and development scientists have tried to develop a more efficient MMLV Reverse transcriptase. After several hard work we have developed a MMLV reverse transcriptase which not only has comparatively better detection capability than Superscript III but also have higher thermal stability. Doing several mutational screening and modification with additional domain we constantly upgrading our reverse transcription enzymes so that we always come up with best product.
 

Specifications

 

 

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Catalogue No. Product Details Datasheet Brochure
G4645 Max MMLV Reverse Transcriptase
G4646 Max MMLV Reverse Transcriptase