Viro XP

Viro XP

Viral RNA Isolation Kit from Buccal swab/Saliva


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ViroXP


Viral RNA Isolation Kit from Buccal swab/Saliva

The ViroXp Viral RNA isolation Kit simplifies purification of viral RNA from body fluids (nasal Swabs, Sputam, bronchoalveolar fluid ) with fast spin-column. A combination of organic extraction and spin column ensure maximum yield with good quality. RNA can be eluted with nuclease free water or buffers.
 

Features

  • Eliminating the risk of viral contamination.
  • Instead of VTM, organic extraction media (kit component) can be directly use for sample storage.
  • Consistent and high yields
  • Complete removal of contaminants

 

Storage

Trizin Reagent store at 4°C & dark and rest store at room temperature.

 

Ordering Information

Cat. No. Product Pack Size Price
GVC101B ViroXP 50 rxn POR
GVC101B2 ViroXP  200 rxn POR

Product Details Specifications Protocols & Manual Product Resource

Description

Trizin® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with Trizin® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA).

The most common method used for extraction of RNA of in an infected person's nasopharyngeal sample is organic extraction. As mentioned before, this method involves isolating the RNA using guanidinium thiocyanate-phenol-chloroform extraction. The chemicals used in this extraction degrade any proteins or cellular components by breaking down the hydrogen bonds within the molecules, whilst protecting DNA and RNA. The RNA is then separated from DNA with an acidic solution consisting of guanidinium thiocyanate, sodium acetate, phenol, and chloroform. After centrifugation, under acidic conditions, the RNA will remain in the upper aqueous phase of the solution, whilst the DNA and proteins remain in the middle and lower organic phase. The total RNA is then precipitated with isopropanol to remove any remaining salts or chemicals on the RNA

SARS-CoV-2, the viral agent that causes the disease, COVID-19, is a positive-sense single stranded RNA virus. Therefore, in order to detect specific RNA molecules, hence specific RNA viruses such as SARS-CoV-2, the RNA molecules must first be converted to DNA molecules for PCR detection.
 
Process work-flow
SARS-CoV-2 is a single-stranded RNA virus. For accurate and sensitive coronavirus detection viral RNA has to be isolated from the sample. So with our ViroXP: Viral RNA Isolation Kit from Buccal swab/Saliva kit you can get pure and high yields of viral RNA molecules. This technique is a combination of Trizin® Reagent, which will precipitate the RNA and efficient glass fiber spin column system is optimized for viral RNA purification from a wide variety of RNA viruses.
 

ViroXP (Viral RNA Isolation Kit from Buccal swab/Saliva)
 

 

Specifications

 

 

Protocol & Manuals

Some product description and more information to download our product brochure or manual datasheet

Catalogue No. Product Details Datasheet Brochure
GVC101B ViroXP
GVC101B2 ViroXP