G-Quant Bradford Reagent
Protein Extraction Buffer
Protein Extraction Buffer delivers high amount of Protein from minimum amount of sample source
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Find support for technicalities of applications and product use
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G-Quant Bradford Reagent
Features
- Ready-to-use dye-binding reagent formulation
- Immediate color development; read at 595nm
- Compatible with reducing substances and chelating agents
- Refrigerated reagent is stable for 12 months
- Determine protein concentration from 100 to 1,500mg/mL
- Convenient microplate or cuvette format
Procedure
The standard protocol can be performed in three different formats, 5 ml and a 1 ml cuvette assay, and a 200 μl microplate assay. The linear range of these assays for BSA is 125–1,000 μg/ml, whereas with bovine gamma globulin (BGG) the linear range is 125–1,500 μg/ml. After removing the container from 4°C to ambient temperature, samples mixer are prepared according to following chart
| Assay | Pack Size | Vol. of 1X Bradford |
| 5 ml | 50 µl | 4950 µl |
| 1ml | 10 µl | 990 µl |
| 0.2 ml | 2 µl | 198 µl |
Samples are incubated for 5 min(it can be extended for up to 60 min)
Description
The G-Quant Bradford assay is a colorimetric protein assay. This assay based on the observation that the absorbance maximum for an acidic solution of coomassie G250 shifts from 465nm to 595nm when binding to protein occour. The Bradford assay is relatively free from interference by most commonly used reducing reagents and biochemical agent upto a certain level. However, a few chemicals may significantly alter the absorbance of the reagent blank or modify the response of proteins to the dye. Basically both hydrophobic and ionic interaction stabilize the anionic form of the dye causing a visible colour change. Therefore materials that stabilize this anionic form of this dye do not support Bradford assay. Materials that are most likely to cause problems in biological extracts are detergents and ampholytes.
G-Quant Bradford Reagent
Ordering Information
| Cat. No. | Product | Pack Size | Price |
| GPQ 01 | G-Quant Bradford Reagent 1X | 500 ml | POR |
| GPQ 02 | G-Quant Bradford Reagent 1X | 1000 ml | POR |
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| GPQ01 | G-Quant Bradford Reagent 1X, 500 ml |  |
|
| GPQ02 | G-Quant Bradford Reagent 1X, 1000 ml | |
|