SARP Stain Protein Dye Gel Staining Kit
Protein Staining Dye
It is difficult to prepare proteins in high amount and desired quality because of the expression and purification challenges in most cases.
- GSure® DNA Extraction & Purification Kit
- GSure® DNA Extraction & Purification Reagents
- Gel Loading Dyes
- DNA Ladder
- Gel Stain
- Running Buffer
- Agarose
Find support for technicalities of applications and product use
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SARP Stain Protein Dye, 100 Small Gel equivalent
Features
- Simple staining, No overstaining
- Delivers a linear quantitation range
- Less protein to protein variability
- Stains all kinds of proteins
- Same gel can be used for blotting or other downstream applications
Ordering Information
|
Cat. No. |
Product | Pack Size | Price |
|
GPS101 |
SARP Stain Protein Dye, 100 Small Gel equivalent | 60 ml | |
| GPS102 | SARP Stain Protein Dye, 250 Small Gel equivalent | 150 ml |
Product Details Specifications Protocols & Manual Product Resource
After STAIN BLOT the same Gel
SDS-PAGE is one of the most powerful and commonly used techniques in molecular biology. However, traditional protein gel staining and destaining methods take several hours to finish and leave a high background on the gel reducing the detection sensitivity. SARP Stain 25 Minutes Gel Staining Solution provides a fast and sensitive method for regular SDS-PAGE gel staining. The procedure requires only 15 minutes for gel sensitization and 10 minutes for staining. Protein Gel stained with SARP stains are readily visualized using UV or Blue light sources. A total of 25-30 minutes allow visualization of 50-100 ng of protein upon UV excitation.
Staining Procedure
SARP staining needs a Glass container and orbital shaker only for better staining. Fixation step is optional. Gels should be sensitized before staining with freshly prepared sensitizing solutions at least for 2 times. After sensitization gels are left in staining solution for next 10 mins. Now gel is ready for documentation with the aid of UV transilluminator. in any gel DOC apparatus (e.g Biorad XRS+). Packs contain sufficient solutions to stain up to 100 /250 pieces of protein mini gels. Working stock of sensitizing solution and staining solution are needed to be prepared freshly with supplied SARP staining reagent.
SARP Stain Protein Dye Gel Staining Kit
Note:
- As the fluorescent dye has a propensity to absorb on plastic surfaces, use of plastic container for staining is not recommended.
- For Blotting equilibrations of the gel in 0.1% SDS containing transfer buffer for 10 -30 min is mandatory for native PAGE.
Protocol & Manuals
Some product description and more information to download our product brochure or manual datasheet
| Catalogue No. | Product Details | Datasheet | Brochure |
| GPS101 | SARP Stain Protein Dye, 100 Small Gel equivalent, 60 ml |  |
|
| GPS102 | SARP Stain Protein Dye, 100 Small Gel equivalent, 150 ml | |
|